ylyB

168

similar to pseudouridylate synthase

locus: BSU_15460

Molecular weight: 33.50 kDa

pI: 5.43

Protein length: 303 aa

Gene length: 912 bp

function: RNA modification

product: putative pseudouridylate synthase

essential: no

ec: 5.4.99.23

synonyms: ylyB, ylmL

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

Gene

Coordinates: 1,617,210 → 1,618,121

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The protein

Catalyzed reaction/ biological activity

• uridine in RNA --> pseudouridine in RNA (according to UniProt)

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RluA family (with [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO] and [protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT], according to UniProt)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 15-74) (according to UniProt)

Structure

• [PDB|1V9F] (RluD from E. coli, 42% identical) [pubmed|15078091]
• [AF|Q45480]

Paralogous protein(s)

• [protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT], [protein|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]

Expression and Regulation

Operons

• genes: [gene|8FC1A0A6C9975F6C125490FBAAB2A4957210A9F1|lspA]-[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]-[gene|6D9CEDC7737CCFA838EBC142CD735E066C950AD2|pyrR]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT,  downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT
• BKK15460 (Δ[gene|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|ylyB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGCGGTTATATCAATTTGAT,  downstream forward: _UP4_TGACAGAGGGTTTCTTTTCT

References

• 17188032,15078091