ylaC

168

RNA polymerase ECF-type sigma factor YlaC

Locus: BSU_14730

Molecular weight: 20.78 kDa

Isoelectric point: 9.28

Protein length: 173 aa

Gene length: 522 bp

Function: Transcription

Product: RNA polymerase ECF-type sigma factor YlaC

Essential: no

Synonyms: ylaC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

Gene

Coordinates: 1,544,085  1,544,606

The protein

Protein family

• Sigma-70 factor family (according to UniProt)
• ECF subfamily (according to UniProt)

Structure

• 5WUQ (PDB) (SigW in complex with the cytoplasmic domain of RsiW, 33% identity) PubMed
• O07627 (AlphaFold)

Effectors of protein activity

• YlaD acts as anti-sigma PubMed

Expression and Regulation

Operons

• Genes: ylaA-ylaB-ylaC-ylaD

  Description: PubMed

  Regulation

  • Spx: transcription activation PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • YlaC: sigma factor, PubMed, in ylaC regulon

• Genes: ylaC-ylaD

  Description: PubMed

  Regulation

  • Spx: transcription activation PubMed

Biological materials

Mutant

• MGNA-B356 (ylaC::erm), available at the NBRP B. subtilis, Japan
• 1A908 ( ylaC::kan), PubMed, available at BGSC
• BKE14730 (ylaC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCAATGGAATCCCTATGCT,  downstream forward: _UP4_AAAAGTAGAGAGGAGGAGCG
• BKK14730 (ylaC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCAATGGAATCCCTATGCT,  downstream forward: _UP4_AAAAGTAGAGAGGAGGAGCG

References

Reviews

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Original Publications

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