ykuE

168

Mn/ Zn-dependent phosphatase

locus: BSU_14050

Molecular weight: 31.61 kDa

pI: 8.89

Protein length: 287 aa

Gene length: 861 bp

function: unknown

product: Mn/ Zn-dependent phosphatase

essential: no

synonyms: ykuE

List of homologs in different organisms, belongs to COG1408 (Galperin et al., 2021)

Gene

Coordinates: 1,477,069 → 1,477,929

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The protein

Protein family

• [wiki|Metallophosphoesterase superfamily] (according to UniProt)

Structure

• [AF|O34870]

[wiki|Localization]

• cell wall, exported by [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY], attached to the wall by electrostatic interactions [Pubmed|22767609]
• [protein|780FDF3DD260287428B397FFE7F44DDF1917381C|tatAY]-[protein|E60FC9C9B273DD6B07B7B95819525DE5F35113CE|tatCY]-dependent export of [protein|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE] requires a functional [protein|DC0FDCA3FA6742B023E6877CE9554AA1D47012BB|wprA] [Pubmed|23180473]

Expression and Regulation

Operons

• genes: [gene|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE]-[gene|6F256986E591DB82974A7F54EB1CE0C024A6F63B|ldt]-[gene|DDAE68CD8D7F0D9C46E62D0EDBF4631C9EC4C236|ykuC]

  description: [Pubmed|22383849]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|11011148]

Biological materials

Mutant

• MGNA-A767 (ykuE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/767 NBRP B. subtilis, Japan]
• BKE14050 (Δ[gene|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCAAACCAACCTTTAA,  downstream forward: _UP4_TAATGTAGCAGGATGGGCTC
• BKK14050 (Δ[gene|253A24EEA02C07E577A96FB64DF39F9A3D0C3A52|ykuE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14050 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCAAACCAACCTTTAA,  downstream forward: _UP4_TAATGTAGCAGGATGGGCTC

References

Reviews

• 24140208

Original Publications

• 22767609,22383849