ykgA

168

similar to arginine-guanidine removing enzyme, general stress protein, survival of salt and ethanol stresses

locus: BSU_13020

Molecular weight: 29.49 kDa

pI: 4.83

Protein length: 286 aa

Gene length: 861 bp

function: survival of salt and ethanol stresses

product: unknown

essential: no

ec: null

synonyms: ykgA

List of homologs in different organisms, belongs to COG1834 (Galperin et al., 2021)

Gene

Coordinates: 1,371,015 → 1,371,875

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The protein

Protein family

• DDAH family (single member, according to UniProt)

Structure

• [PDB|6LRG] (arginine-guanidine removing enzyme from Escherichia coli, corresponds to aa 23 ... 280, 26% identity) [pubmed|32198136]
• [AF|O34497]

Expression and Regulation

Operons

• genes: [gene|E8ECEFF86ABD1A2BE439F3CAD5A7C5BB58C4BF8E|ykgA]-[gene|491B5E27EF3190D307EC43C3BB12490728E39333|ykgB]

  description: [Pubmed|10482513]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|10482513]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|10482513], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-A753 (ykgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/753 NBRP B. subtilis, Japan]
• BKE13020 (Δ[gene|E8ECEFF86ABD1A2BE439F3CAD5A7C5BB58C4BF8E|ykgA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTTTCCTCCTAAA,  downstream forward: _UP4_TGAAGAATCTTCCCCTTTTT
• BKK13020 (Δ[gene|E8ECEFF86ABD1A2BE439F3CAD5A7C5BB58C4BF8E|ykgA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTTTCCTCCTAAA,  downstream forward: _UP4_TGAAGAATCTTCCCCTTTTT

References

• 15805528,10482513,32198136