ykcC

168

lipoteichoic acid glycosyltransferase, generation of C₅₅-P-Glc

locus: BSU_12890

Molecular weight: 37.22 kDa

pI: 8.01

Protein length: 323 aa

Gene length: 972 bp

function: glycosylation of teichoic acids

product: glycosyltransferase

essential: no

synonyms: ykcC

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 1,356,447 → 1,357,418

Advertisement

The protein

Protein family

• [wiki|glycosyltransferase 2 family] (according to UniProt)

Structure

• [PDB|5EKP] (from Synechocystis sp., 47% identity) [pubmed|26729507]
• [AF|O34319]

Paralogous protein(s)

• [protein|824E6AE2FFA3CBC9F90648F15178A00A6CC49E4C|csbB]
• [protein|EF18470AD2B07755431534B52A8FCE6754FDBA9D|ykoT], (39%)

[wiki|Localization]

• cell membrane (heterogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|7039FCF49F97FFEF37ED48D6F860E3873A5CF308|ykcB]-[gene|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|ykcC]

  description: [Pubmed|18175906]

  regulatory mechanism

  • [protein|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]: activation, [Pubmed|20512483], in [regulon|protein:706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ regulon]
  • [protein|1582F9E6510C4FA87B3A5F3F4A7F39008C654AA9|yrkP]: activation, [Pubmed|18175906], in [regulon|protein:1582F9E6510C4FA87B3A5F3F4A7F39008C654AA9|yrkP regulon]

Biological materials

Mutant

• MGNA-A739 (ykcC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/739 NBRP B. subtilis, Japan]
• BKE12890 (Δ[gene|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|ykcC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATGTCTGCTCATGTTTTTGC,  downstream forward: _UP4_TAACCTCAAACCCCCTGTCC
• BKK12890 (Δ[gene|FD76A781162795EAF174B59C0A90D2ED3ACC0AB7|ykcC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATGTCTGCTCATGTTTTTGC,  downstream forward: _UP4_TAACCTCAAACCCCCTGTCC

References

• 20512483,18175906,16479537,21630458,27185829,26729507,36409129