yjoB

168

putative substrate-specific molecular chaperone, prevents [protein|C1B89BD3DD27CD566018558D3A7845FB451056F0|gapB] and [protein|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA] from aggregation at elevated temperatures, AAA family, ATPase activity , similar to cell-division protein [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

locus: BSU_12420

Molecular weight: 48.61 kDa

pI: 4.73

Protein length: 423 aa

Gene length: 1272 bp

function: unknown

product: putative substrate-specific molecular chaperone

essential: no

synonyms: yjoB

List of homologs in different organisms, belongs to COG0465 (Galperin et al., 2021)

Gene

Coordinates: 1,314,453 → 1,315,724

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The protein

Protein family

• [wiki|AAA ATPase family] (according to UniProt)

Structure

• [PDB|7W42] [pubmed|36191235]
• [PDB|7W43] (N-terminal domain) [pubmed|36191235]
• [PDB|7W46] (complex with ADP) [pubmed|36191235]
• [PDB|1LV7] (AAA domain of E. coli [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH], correponds to aa 210 ... 400 of YjoB, 32% identity) [pubmed|12176385]
• [AF|O34703]

Paralogous protein(s)

• [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH]

Expression and Regulation

Operons

• genes: [gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]

  description: [Pubmed|1378051]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|9987136], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]

Biological materials

Mutant

• GP3785 (Δ[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]::neo) available in [wiki|Jörg Stülke]'s lab
• MGNA-A031 (yjoB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/31 NBRP B. subtilis, Japan]
• BKE12420 (Δ[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATACTCCATTTCA,  downstream forward: _UP4_TAAAAGAAAGCACGGGTGTT
• BKK12420 (Δ[gene|E278CCD5CFD618A0D4ACB92E1E06DCC1B4570ED0|yjoB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATACTCCATTTCA,  downstream forward: _UP4_TAAAAGAAAGCACGGGTGTT

References

• 14757246,1378051,9987136,12176385,36191235