yjiB

168

cytochrome P450, monooxygenase CYP109B1

locus: BSU_12210

Molecular weight: 44.83 kDa

pI: 5.38

Protein length: 396 aa

Gene length: 1191 bp

function: oxidation of fatty acids

product: monooxygenase CYP109B1

essential: no

synonyms: yjiB

List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene

Coordinates: 1,291,344 → 1,292,534

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The protein

Catalyzed reaction/ biological activity

• oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group [Pubmed|20186410]

Protein family

• [wiki|cytochrome P450] family (according to UniProt)

Structure

• [PDB|4RM4] [Pubmed|25587700]
• [AF|O34374]

Paralogous protein(s)

• [protein|EF0F13BB682791E167EFC350BF029B08E8A1F410|pksS], [protein|A95C28DEE73AD25E28C45B88A7C03C92835F9705|cypA], [protein|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]

Expression and Regulation

Operons

• genes: [gene|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|yjiB]-[gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]

  description: [pubmed|22383849]

  regulation

  • induced by stress ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]) [pubmed|26577401]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [pubmed|26577401], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-A349 (yjiB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/349 NBRP B. subtilis, Japan]
• BKE12210 (Δ[gene|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|yjiB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACGGTCACTCCCCTT,  downstream forward: _UP4_TAATAGATAAGGAGACTGGA
• BKK12210 (Δ[gene|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|yjiB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACGGTCACTCCCCTT,  downstream forward: _UP4_TAATAGATAAGGAGACTGGA

References

• 19591681,20186410,25587700,22383849