yitU

168

5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

locus: BSU_11140

Molecular weight: 30.48 kDa

pI: 4.67

Protein length: 270 aa

Gene length: 813 bp

function: dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity)

product: 5'-nucleotidase

essential: no

synonyms: yitU

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 1,190,490  1,191,302

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The protein

Catalyzed reaction/ biological activity

• dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate (minor activity) [Pubmed|26316208]
• 5-amino-6-(5-phospho-D-ribitylamino)uracil + H₂O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)

Protein family

• [wiki|HAD superfamily] (according to UniProt) [Pubmed|26316208]
• [wiki|Cof family] (according to UniProt)

Structure

• [PDB|1RKQ] (YidA from E. coli, 31% identity)
• [AF|P70947]

Expression and Regulation

Operons

• genes: [gene|29EFA7585D7B2FD3A2CDBE2F21AB6742980759B4|yitU]-[gene|8A1EEBB7F3CA4ADE6C1D9ADACFC00DCEF29C3304|yizC]

  description: [Pubmed|22383849]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [pubmed|30782632], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-B185 (yitU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1184 NBRP B. subtilis, Japan]
• BKE11140 ([gene|29EFA7585D7B2FD3A2CDBE2F21AB6742980759B4|yitU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAAGCTCCTTTAACC,  downstream forward: _UP4_TAAAGAAGGTCCGTATTAAT
• BKK11140 ([gene|29EFA7585D7B2FD3A2CDBE2F21AB6742980759B4|yitU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11140 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAAAGCTCCTTTAACC,  downstream forward: _UP4_TAAAGAAGGTCCGTATTAAT

References

• 26316208,32040605