yitF

168

similar to mandelate racemase

locus: BSU_10970

Molecular weight: 41.89 kDa

pI: 7.53

Protein length: 371 aa

Gene length: 1116 bp

function: unknown

product: unknown

essential: no

ec: null

synonyms: yitF

List of homologs in different organisms, belongs to COG4948 (Galperin et al., 2021)

Gene

Coordinates: 1,174,861 → 1,175,976

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The protein

Protein family

• [wiki|mandelate racemase/muconate lactonizing enzyme family] (according to UniProt)

Structure

• [PDB|2GDQ]
• [AF|O06741]

Expression and Regulation

Operons

• genes: [gene|BA1A7ECAE0B5690F6E6A67785BAB18628701E2A2|yitG]-[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]

  description: [Pubmed|16497325]

  regulation

  • expressed during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]) [Pubmed|16497325,15699190]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|16497325,15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• MGNA-B180 (yitF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1179 NBRP B. subtilis, Japan]
• BKE10970 (Δ[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCGTACAATTTTCACACTGG,  downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA
• BKK10970 (Δ[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCGTACAATTTTCACACTGG,  downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA

References

• 16497325,15699190