yitB

yitB
168

phospho-adenylylsulfate sulfotransferase

locus
BSU_10930
Molecular weight
27.52 kDa
pI
5.77
Protein length
Gene length
function
sulfate reduction and activation
product
phospho-adenylylsulfate sulfotransferase
essential
no
ec
1.8.4.8
synonyms
yitB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0175 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,171,755 → 1,172,465
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The protein
Catalyzed reaction/ biological activity
[thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H+ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)
Protein family
PAPS reductase family (with [protein|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH], according to UniProt)
Structure
[PDB|2OQ2] (from yeast, 33% identity) [pubmed|18991405]
[AF|O06737]
Paralogous protein(s)
[protein|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]-[gene|EBBA4432FF83B6F203C694E04111D45290BE157A|yitA]-[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]
description
[Pubmed|22383849]
regulation
expressed during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,15383836]
sigma factors
[protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190,15383836], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]
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[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]→[gene|FE2B31E5EBE6C4BA4FC4AAC485A00C97315E6AAB|yisZ]

2024-01-19 21:55:12

ghost

51

4fca3e0835090887f1ca8f30217e3a4a8ad039c1

C607370DEEFC425F067119B36C7AC0A95C3ADF71

Biological materials
Mutant
MGNA-A504 (yitB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/504 NBRP B. subtilis, Japan]
BKE10930 (Δ[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG,  downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
BKK10930 (Δ[gene|E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E|yitB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10930 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG,  downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
References
15699190,15383836,18991405

E5090587E23B284EF3B3D8F3EA5C44CB86EEB82E

Page visits: 2179

Time of last update: 2024-03-28 10:08:38

Author of last update: Melvin.boenninger