yisV

168

similar to transcriptional regulator ([wiki|MocR/ GabR family])

locus: BSU_10880

Molecular weight: 54.11 kDa

pI: 9.3

Protein length: 484 aa

Gene length: 1455 bp

function: unknown

product: unknown

essential: no

synonyms: yisV, yisW

List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 1,166,737  1,168,191

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The protein

Protein family

• [wiki|MocR/ GabR family] [Pubmed|22020104]
• [wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• [wiki|HTH gntR-type domain] (aa 14-82) (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|1WST] (aminotransferase from Thermococcus profundus, 29% identity)
• [AF|Q796Q6]

Paralogous protein(s)

• [protein|B1A95EAE40AFA40BF12EE9B41287921417A39562|ydfD]

Expression and Regulation

Operons

• genes: [gene|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|yisV]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B211 (yisW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1210 NBRP B. subtilis, Japan]
• BKE10880 ([gene|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|yisV]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCCCCCTTTTTTACAC,  downstream forward: _UP4_CGCCTGATGATGTCCTAGGC
• BKK10880 ([gene|6A3F08CD939CCDEC1F784D658B815A6D0E1AAC3D|yisV]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCCCCCTTTTTTACAC,  downstream forward: _UP4_CGCCTGATGATGTCCTAGGC

References

• 11756427,22020104