yhdI

168

similar to transcriptional regulator ([wiki|MocR/ GabR family])

locus: BSU_09480

Molecular weight: 52.60 kDa

pI: 6.8

Protein length: 469 aa

Gene length: 1410 bp

function: unknown

product: unknown

essential: no

synonyms: yhdI

List of homologs in different organisms, belongs to COG1167 (Galperin et al., 2021)

Gene

Coordinates: 1,026,255 → 1,027,664

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The protein

Protein family

• [wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
• [wiki|MocR/ GabR family ][Pubmed|22020104]

[wiki|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• [wiki|HTH gntR-type domain] (aa 13-81) (according to UniProt)

[wiki|Cofactors]

• PLP

Structure

• [PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|gabR], 40% identity) [pubmed|24127574]
• [AF|O07578]

Expression and Regulation

Operons

• genes: [gene|D0498CC264FFC4B4C0DECAEBBBB884DB1122A4B2|yhdI]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B480 (yhdI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1479 NBRP B. subtilis, Japan]
• BKE09480 (Δ[gene|D0498CC264FFC4B4C0DECAEBBBB884DB1122A4B2|yhdI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCATGTCTCCTTTGTA,  downstream forward: _UP4_TAAATAAAAAACAGGCCCCG
• BKK09480 (Δ[gene|D0498CC264FFC4B4C0DECAEBBBB884DB1122A4B2|yhdI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCATGTCTCCTTTGTA,  downstream forward: _UP4_TAAATAAAAAACAGGCCCCG

References

• 11756427,22020104,24127574