yhdA

168

NADPH-dependent FMN oxidoreductase

locus: BSU_09340

Molecular weight: 18.76 kDa

pI: 6.51

Protein length: 174 aa

Gene length: 525 bp

function: protection against oxidative stress

product: NADPH-dependent FMN oxidoreductase

essential: no

synonyms: yhdA, azr

List of homologs in different organisms, belongs to COG0431 (Galperin et al., 2021)

Gene

Coordinates: 1,010,445  1,010,969

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The protein

Protein family

• azoreductase type 2 family (single member, according to UniProt)

[wiki|Cofactors]

• FMN [Pubmed|16752898]
• NADPH [pubmed|32801174]

Structure

• [PDB|3GFQ] (99% identity),
• [PDB|1NNI]
• [AF|O07529]

Paralogous protein(s)

• [protein|CAFAE305B1771DD89E81F75A6713161393BE99A0|pgcM], (31%)

Expression and Regulation

Operons

• genes: [gene|8D570AEE5D78A9A3CDBB1F457630BAF5DB59BCFD|yhcY]-[gene|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ]-[gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]

  description: [Pubmed|16816187]

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]: activation, [Pubmed|16816187], in [regulon|protein:49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16816187], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A689 (yhdA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/689 NBRP B. subtilis, Japan]
• BKE09340 ([gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGTGCCATTTATGACTAACA,  downstream forward: _UP4_GGCGTCTAAACGCCGGGATT
• BKK09340 ([gene|345EC248C0ADF48B0C25B175241AAE982E0E9B78|yhdA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09340 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGTGCCATTTATGACTAACA,  downstream forward: _UP4_GGCGTCTAAACGCCGGGATT

References

• 16816187,16752898,20639339,20640807,32801174,33289153