yhcG

168

similar to [wiki|ABC transporter] (ATP-binding protein)

locus: BSU_09070

Molecular weight: 26.37 kDa

pI: 5.36

Protein length: 232 aa

Gene length: 699 bp

function: unknown

product: [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: yhcG

List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

Gene

Coordinates: 981,604 → 982,302

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 3-226) (according to UniProt)

Structure

• [PDB|5X5Y] (from Pseudomonas aeruginosa, 30% identity) [pubmed|28394325]
• [AF|P54591]

[wiki|Localization]

• membrane (via [protein|74B0D594308D313A54C7163759628655382B1F94|yhcE]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|74B0D594308D313A54C7163759628655382B1F94|yhcE]-[gene|53E29A238EC817CE9B430BDED92DE8E9746C928B|yhcF]-[gene|E71E305A0DE5AB6BE24CE47DC1ECCD99670C24D8|yhcG]-[gene|70858AED096A80C1EAEB81F4B048368B665AB515|yhcH]-[gene|BAD93C5EAD25F1A257907F4C631C02FE97C7BC89|yhcI]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A659 (yhcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/659 NBRP B. subtilis, Japan]
• GP2265 (''[gene|E71E305A0DE5AB6BE24CE47DC1ECCD99670C24D8|yhcG]''::''ermC''), available in [wiki|Jörg Stülke]'s lab
• BKE09070 (Δ[gene|E71E305A0DE5AB6BE24CE47DC1ECCD99670C24D8|yhcG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACATGTTCTAGCTTGATTT,  downstream forward: _UP4_GTTTGCTAGAAAGAGGAGGA
• BKK09070 (Δ[gene|E71E305A0DE5AB6BE24CE47DC1ECCD99670C24D8|yhcG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09070 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TACATGTTCTAGCTTGATTT,  downstream forward: _UP4_GTTTGCTAGAAAGAGGAGGA

References

• 10092453,21815947,28394325