ygaK

168

uncharacterized FAD-linked oxidoreductase

locus: BSU_08800

Molecular weight: 40.96 kDa

pI: 7.12

Protein length: 451 aa

Gene length: 1356 bp

function: unknown

product: unknown

essential: no

synonyms: ygaK

List of homologs in different organisms, belongs to COG0277 (Galperin et al., 2021)

Gene

Coordinates: 957,705  959,060

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The protein

Protein family

• oxygen-dependent FAD-linked oxidoreductase family (with [protein|23F2385D14DDD5C400DC897E0F0FC814ACFC36B8|yitY] and [protein|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ], according to UniProt)

[wiki|Domains]

• [wiki|FAD-binding PCMH-type domain] (aa 29-204) (according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|3W8W] (from ''Streptomyces maritimus'', 33%)
• [AF|Q796Y5]

Paralogous protein(s)

• [protein|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ], (40%)

Expression and Regulation

Operons

• genes: [gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]

  description: [Pubmed|22383849]

  regulation

  • expression during spore [wiki|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-A246 (ygaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/246 NBRP B. subtilis, Japan]
• BKE08800 ([gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACTCCCACATCTCTTTC,  downstream forward: _UP4_TAAAAAAATGAAGATGGAGC
• BKK08800 ([gene|11499CDC836A40E857B8F5AD746BA2C650DC916B|ygaK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACTCCCACATCTCTTTC,  downstream forward: _UP4_TAAAAAAATGAAGATGGAGC

References

• 22383849,27766092