yfkQ

168

part of the [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]-[protein|B7EEC1910715507596734540D24B3C93B4B7065F|yfkR]-[protein|7A62875A659B9F0967C392F38895D9E15A29D559|yfkT] germinant receptor of unknown specificity

locus: BSU_07790

Molecular weight: 57.54 kDa

pI: 6.23

Protein length: 513 aa

Gene length: 1542 bp

function: germination

product: part of the [protein|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]-[protein|B7EEC1910715507596734540D24B3C93B4B7065F|yfkR]-[protein|7A62875A659B9F0967C392F38895D9E15A29D559|yfkT] germinant receptor

essential: no

synonyms: yfkQ

List of homologs in different organisms, belongs to COG5901 (Galperin et al., 2021)

Gene

Coordinates: 848,633  850,174

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The protein

Protein family

• [wiki|GerABKA family] (according to UniProt)

Structure

• [PDB|6O59] (from B. megaterium, corresponds to aa 31 ... 281, 44.1% identity) [pubmed|31113879]
• [AF|O34486]

Paralogous protein(s)

• [protein|AE28299B0F274B189DD90554A5A4F7B9A9479B60|yndD], [protein|EDB2F4C132211F01654F2A2ED3D220EC15CE6F22|gerKA], [protein|EEDE6A22E91992ABCA677CAB7917B7168C5CB868|gerAA], [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]-[gene|B7EEC1910715507596734540D24B3C93B4B7065F|yfkR]-[gene|AA76BEF6A4CECB5D3910918E8D91D8B919EDEA7E|yfkS]-[gene|7A62875A659B9F0967C392F38895D9E15A29D559|yfkT]

  description: [Pubmed|22383849]

  regulation

  • expressed during [wiki|sporulation] in the forespore [Pubmed|15699190]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-C345 (yfkQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2343 NBRP B. subtilis, Japan]
• BKE07790 ([gene|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGAGGCTCACCCTGCTT,  downstream forward: _UP4_GGAGCGAAAAGAGAGGACCC
• BKK07790 ([gene|7CE0CAF27B1C0F507E783B8B80622270B00634FD|yfkQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07790 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGAGGCTCACCCTGCTT,  downstream forward: _UP4_GGAGCGAAAAGAGAGGACCC

References

• 15699190,22383849,10762253,31113879