yfiR

168

transcriptional regulator ([wiki|TetR family]) of the [gene|09D56215645F3E13803704F96FC6AD3FAAD58EF4|yfiS]-[gene|search|yfiR ]operon

locus: BSU_08370

Molecular weight: 23.54 kDa

pI: 5.02

Protein length: 205 aa

Gene length: 618 bp

function: control of [gene|search|yfiS ]expression

product: transcriptional regulator ([wiki|TetR family])

essential: no

synonyms: yfiR

List of homologs in different organisms, belongs to COG1309 (Galperin et al., 2021)

Gene

Coordinates: 911,964  912,581

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The protein

Protein family

• [wiki|TetR family]

[wiki|Domains]

• [wiki|HTH tetR-type domain] (aa 11-71) (according to UniProt)

Structure

• [PDB|1RKT] [Pubmed|16862575]
• [AF|O31560]

Expression and Regulation

Operons

• genes: [gene|09D56215645F3E13803704F96FC6AD3FAAD58EF4|yfiS]-[gene|15DE8BAFB36296B5842E283C836D600755786397|yfiR]

  description: (according to [http://dbtbs.hgc.jp/COG/prom/yfiSR.html DBTBS]) null

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|25755103], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
  • [protein|15DE8BAFB36296B5842E283C836D600755786397|yfiR]: repression, [pubmed|], in [regulon|protein:15DE8BAFB36296B5842E283C836D600755786397|yfiR regulon]

• genes: [gene|15DE8BAFB36296B5842E283C836D600755786397|yfiR]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C356 (yfiR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2354 NBRP B. subtilis, Japan]
• BKE08370 ([gene|15DE8BAFB36296B5842E283C836D600755786397|yfiR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTTATCCTTGTGCTCCTTT,  downstream forward: _UP4_TAAAGAAACGCGGCAGGAGC
• BKK08370 ([gene|15DE8BAFB36296B5842E283C836D600755786397|yfiR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTTATCCTTGTGCTCCTTT,  downstream forward: _UP4_TAAAGAAACGCGGCAGGAGC

References

Reviews

• 24006471

Original Publications

• 25755103,16862575,22383849