yetM

168

FAD-dependent monooxygenase

locus: BSU_07230

Molecular weight: 40.82 kDa

pI: 5.34

Protein length: 369 aa

Gene length: 1110 bp

function: unknown

product: FAD-dependent monooxygenase

essential: no

synonyms: yetM

List of homologs in different organisms, belongs to COG0654 (Galperin et al., 2021)

Gene

Coordinates: 790,318  791,427

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The protein

[wiki|Cofactors]

• FAD (according to UniProt) [Pubmed|21635694]

Structure

• [PDB|4H2N] (from Mesorhizobium japonicum, 30% identity)
• [AF|O06489]

Expression and Regulation

Operons

• genes: [gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM]

  description: [Pubmed|19329649]

  regulation

  • induced by flavonoids of kaempferol, apigenin, and luteolin ([protein|search|YetL])[Pubmed|19329649]

  regulatory mechanism

  • [protein|BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|yetL]: repression, [Pubmed|19329649], in [regulon|protein:BFAFC2CFA7F2FDA639B367C579E2A46242BA3DBD|yetL regulon]

Biological materials

Mutant

• MGNA-B464 (yetM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1463 NBRP B. subtilis, Japan]
• BKE07230 ([gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAATAACCACCTTTT,  downstream forward: _UP4_TAAATAGGAAAAGTCCAGAA
• BKK07230 ([gene|A70EA4AEBF46ED2279E8BCE8A28439078484B6E3|yetM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07230 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCAATAACCACCTTTT,  downstream forward: _UP4_TAAATAGGAAAAGTCCAGAA

References

• 19329649,19329649