ydjM

168

member of the [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon], putative cell wall hydrolase (N-acetylglucosamidase)

locus: BSU_06250

Molecular weight: 13.04 kDa

pI: 10.57

Protein length: 123 aa

Gene length: 372 bp

function: may be involved in cell wall metabolism ([regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon])

product: unknown

essential: no

synonyms: ydjM, yzvA

List of homologs in different organisms

Gene

Coordinates: 679,390  679,761

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The protein

Structure

• [AF|P40775]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|20709850]

Expression and Regulation

Operons

• genes: [gene|18ED48E99D87748B2EBD6001EFDF1D3D91535ABF|ydjM]

  description: [Pubmed|22383849]

  regulation

  • activated by [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] [Pubmed|17581128]

  regulatory mechanism

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|17581128], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|20059685], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]

  additional information

  • the terminator is [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]-dependent [ http://www.nature.com/articles/nmicrobiol20157 Reference]

• genes: [gene|18ED48E99D87748B2EBD6001EFDF1D3D91535ABF|ydjM]-[gene|6B93DA610D4D86A5D4E53190999FB0E4E10AE8E5|ydjN]

  description: [Pubmed|22383849]

  regulation

  • activated by [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR] [Pubmed|17581128]

  regulatory mechanism

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [pubmed|17581128], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [pubmed|20059685], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]

  additional information

  • the terminator is [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]-dependent [ http://www.nature.com/articles/nmicrobiol20157 Reference]

Biological materials

Mutant

• MGNA-C220 (ydjM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2218 NBRP B. subtilis, Japan]
• BKE06250 ([gene|18ED48E99D87748B2EBD6001EFDF1D3D91535ABF|ydjM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTCCCCCTGTTTGT,  downstream forward: _UP4_TAATGTACATATATAATCTG
• BKK06250 ([gene|18ED48E99D87748B2EBD6001EFDF1D3D91535ABF|ydjM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06250 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCGTCCCCCTGTTTGT,  downstream forward: _UP4_TAATGTACATATATAATCTG

References

• 17581128,20059685,20709850,23199363