ydjI

168

required for membrane localization of [protein|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA] (together with [protein|F93381205C61665820D034A384BD60410AAE84C1|ydjG] and [protein|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH])

locus: BSU_06210

Molecular weight: 35.92 kDa

pI: 4.94

Protein length: 323 aa

Gene length: 972 bp

function: targeting of [protein|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]

product: unknown

essential: no

ec: null

synonyms: ydjI

List of homologs in different organisms, belongs to COG4260 (Galperin et al., 2021)

Gene

Coordinates: 673,814 → 674,785

Phenotypes of a mutant

• reduced membrane fluidity [pubmed|34777311]

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The protein

Catalyzed reaction/ biological activity

• membrane localization of [protein|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA] (together with [protein|F93381205C61665820D034A384BD60410AAE84C1|ydjG] and [protein|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH]) [pubmed|34777311]

Protein family

• Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) domain protein family (with [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA] and [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT])

[wiki|Domains]

• contains a SPFH domain (Stomatin, Prohibitin, Flotillin, and HflK/C-Domain) (aa 22 - 232) [pubmed|34777311]
• C-terminal zinc-finger domain (aa 300 - 322) [pubmed|34777311]

Structure

• [AF|O34789]

[wiki|Localization]

• cell membrane (fluid regions) (via [protein|F93381205C61665820D034A384BD60410AAE84C1|ydjG] and [protein|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH])) [pubmed|34777311]

Expression and Regulation

Operons

• genes: [gene|3B6F4FBB19401069E827EAF5835DD15AAA3C3487|pspA]-[gene|F93381205C61665820D034A384BD60410AAE84C1|ydjG]-[gene|938B55332DFB2B465D0D5AD530550CED5AC30A92|ydjH]-[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]

  description: [Pubmed|11454200]

  regulation

  • induced by alkaline shock ([protein|search|SigW]) [Pubmed|11454200]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|18840696,20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11454200], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]

Biological materials

Mutant

• MGNA-C216 (ydjI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2214 NBRP B. subtilis, Japan]
• BKE06210 (Δ[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACGCCTCTCCCTTTCT,  downstream forward: _UP4_TAAGAATCATACAGTGAAAA
• BKK06210 (Δ[gene|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACGCCTCTCCCTTTCT,  downstream forward: _UP4_TAAGAATCATACAGTGAAAA

References

• 11454200,20817675,34777311,35604119