ycsE

168

5'-nucleotidase, dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate

locus: BSU_04040

Molecular weight: 27.92 kDa

pI: 4.49

Protein length: 249 aa

Gene length: 750 bp

function: dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate

product: 5'-nucleotidase

essential: no

synonyms: ycsE

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 456,068 → 456,817

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The protein

Catalyzed reaction/ biological activity

• dephosphorylation of the riboflavin precursor, 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate [Pubmed|26316208]
• 5-amino-6-(5-phospho-D-ribitylamino)uracil + H₂O --> 5-amino-6-(D-ribitylamino)uracil + phosphate (according to UniProt)

Protein family

• [wiki|HAD superfamily] (according to UniProt) [Pubmed|26316208]
• [wiki|Cof family] (according to UniProt)

Structure

• [PDB|1NRW] ([protein|AF9523D3AA3C032B70175BCFFED34E9A42ED01AF|phoC], 31% identity)
• [AF|P42962]

Biological materials

Mutant

• MGNA-C071 (ycsE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2069 NBRP B. subtilis, Japan]
• BKE04040 (Δ[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE04040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATATGTACCTCTCTTTA,  downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG
• BKK04040 (Δ[gene|D8AC0ECC57C6113D0CCA36FC38395EECBD00FF50|ycsE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK04040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATATGTACCTCTCTTTA,  downstream forward: _UP4_TAAAAAAAGAGAGTCCTAAG

References

• 16990279,27784292,26316208,22383849,25848029