ycgS

168

similar to aromatic hydrocarbon catabolism

locus: BSU_03260

Molecular weight: 31.59 kDa

pI: 8.23

Protein length: 284 aa

Gene length: 855 bp

function: unknown

product: unknown

essential: no

synonyms: ycgS

List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 351,842  352,696

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The protein

Protein family

• [wiki|AB hydrolase superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|AB hydrolase-1 domain] (aa 25-123) (according to UniProt)

Structure

• [PDB|4Y7D] (from Nakamurella multipartita, 25% identity)
• [AF|P94396]

Expression and Regulation

Operons

• genes: [gene|1AA67AD33622538F7BAD64B54FAE6FD8103BF9BE|ycgS]-[gene|51EF3C9A48FA7367576656F5E79D38D1B56BF6A5|ycgR]-[gene|99C8AA68035A9580FA3145ABE66F4DD63CDD6AE8|ycgQ]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B997 (ycgS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1996 NBRP B. subtilis, Japan]
• BKE03260 ([gene|1AA67AD33622538F7BAD64B54FAE6FD8103BF9BE|ycgS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTCACCCAATTGG,  downstream forward: _UP4_TAACCAAAGTGAAACGATCC
• BKK03260 ([gene|1AA67AD33622538F7BAD64B54FAE6FD8103BF9BE|ycgS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTCACCCAATTGG,  downstream forward: _UP4_TAACCAAAGTGAAACGATCC