ycbL

168

two-component response regulator ([wiki|OmpR family])

locus: BSU_02550

Molecular weight: 25.66 kDa

pI: 4.92

Protein length: 226 aa

Gene length: 681 bp

function: unknown

product: two-component response regulator ([wiki|OmpR family])

essential: no

synonyms: ycbL

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 278,377  279,057

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The protein

Protein family

• [wiki|OmpR family] of two-component response regulators

[wiki|Domains]

• [wiki|Response regulatory domain] (aa 1-112) (according to UniProt)

Structure

• [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 38% identity) [pubmed|17942407]
• [AF|P42244]

Modification

• phosphorylation by [protein|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM] ( on an aspartate residue, putative)

Effectors of protein activity

• activity is controlled by [protein|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]-dependent phosphorylation (putative)

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|60DA46B02BB5F71337AF5F34921E2E68EC062EC7|ycbL]-[gene|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]-[gene|FE7AC936BE959D745FBB6A88EAD64408C7177125|ycbN]-[gene|621FA6E2A76BB74F9051FBDB6857ED56F65BAB15|ycbO]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C034 (ycbL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2032 NBRP B. subtilis, Japan]
• BKE02550 ([gene|60DA46B02BB5F71337AF5F34921E2E68EC062EC7|ycbL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGCGCTGCATCTCCT,  downstream forward: _UP4_GGTTATAAATTAGGAGAGTT
• BKK02550 ([gene|60DA46B02BB5F71337AF5F34921E2E68EC062EC7|ycbL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGCGCTGCATCTCCT,  downstream forward: _UP4_GGTTATAAATTAGGAGAGTT

References

• 10094672,23504016,17942407