namZ

168

exo-β-N-acetylmuramidase

locus: BSU_01650

Molecular weight: 45.87 kDa

pI: 7.37

Protein length: 414 aa

Gene length: 1245 bp

function: cell wall turnover and recycling

product: exo-β-N-acetylmuramidase

essential: no

synonyms: ybbC, yzbB

List of homologs in different organisms, belongs to COG3876 (Galperin et al., 2021)

Gene

Coordinates: 185,194  186,438

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The protein

Catalyzed reaction/ biological activity

• exo-lytic cleavage of β-1,4-MurNAc entities from the non-reducing ends of peptidoglycan chains [pubmed|33684445]
• hydrolysis of the peptidoglycan-derived disaccharide MurNAc-β-1,4-GlcNAc [pubmed|33684445]

Protein family

• DUF1343 protein family [pubmed|33684445]

Structure

• [PDB|4K05] (from Bacteroides fragilis, 43% identity)
• [AF|P40407]

Expression and Regulation

Operons

• genes: [gene|0B1F43F43A4DC1574A153DBFBAA5D0A1855F9B61|murQ]-[gene|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]-[gene|178D5E2AA1225FE909E6F2B63B3595F5ABB8E7A2|murP]-[gene|DEACFD435DB01C4B3ADEF397BEE08E2E7C7FCC36|amiE]-[gene|A5D4BF262BD365DCEBA9358C8DB295E605DE4D84|nagZ]-[gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|namZ]

  description: [Pubmed|22383849]

  regulation

  • expressed in late exponential and early stationary phase [Pubmed|20400549]

  regulatory mechanism

  • [protein|125872506C400CB6B3DAEA8B5130B064DFBA4494|murR]: repression, [pubmed|30038046], in [regulon|protein:125872506C400CB6B3DAEA8B5130B064DFBA4494|murR regulon]

Biological materials

Mutant

• BKE01650 ([gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|namZ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTTCTCATGTGTCCCT,  downstream forward: _UP4_TAGAACATAAGCATCGTATG
• BKK01650 ([gene|005D37F34CA3D6891F7D8FF7A2AFA1CBCB556FFE|namZ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTTTTCTCATGTGTCCCT,  downstream forward: _UP4_TAGAACATAAGCATCGTATG

References

• 22383849,30038046,33684445