ecfA2

168

ATP-binding A2 component of [wiki|ECF transporter]s

locus: BSU_01460

Molecular weight: 30.43 kDa

pI: 5.82

Protein length: 276 aa

Gene length: 831 bp

function: uptake of micronutrients

product: [wiki|ECF transporter] (ATP-binding protein)

essential: no

ec: 3.6.3.-

synonyms: ybaE

List of homologs in different organisms, belongs to COG1122 (Galperin et al., 2021)

Gene

Coordinates: 151,303  152,133

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 1-233) (according to UniProt)

Structure

• [PDB|4HUQ] (EcfA1-EcfA2-EcfT-FolT complex of ''Lactobacillus brevis''), [Pubmed|23584589]
• [AF|P70970]

Paralogous protein(s)

• [protein|D8637043C7E2291589C966E95F94DC61B1A8AB75|ecfA1]

[wiki|Localization]

• membrane associated (via [protein|7DC7BD54ED425859FFD45B8B6F3522750A938835|ecfT]) [Pubmed|10092453]

Biological materials

Mutant

• BKE01460 ([gene|5ABF4F7A3D00B523F67B281241A6A8101EC60057|ecfA2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGTCTTCATCTGATAGCGAT,  downstream forward: _UP4_CTTTTTCAGGGAGAGAAGAC
• BKK01460 ([gene|5ABF4F7A3D00B523F67B281241A6A8101EC60057|ecfA2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01460 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGTCTTCATCTGATAGCGAT,  downstream forward: _UP4_CTTTTTCAGGGAGAGAAGAC
• BP1149 ([gene|D8637043C7E2291589C966E95F94DC61B1A8AB75|ecfA1]-[gene|5ABF4F7A3D00B523F67B281241A6A8101EC60057|ecfA2]-[gene|7DC7BD54ED425859FFD45B8B6F3522750A938835|ecfT]::cat [gene|search|trp]⁺) available at [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs

References

• 10092453,20497229,22574898,24362466,30485755