yacO

168

putative 23S rRNA 2'-O-ribose G2251 methyltransferase

locus: BSU_00960

Molecular weight: 27.36 kDa

pI: 8.63

Protein length: 249 aa

Gene length: 750 bp

function: rRNA modification

product: putative 23S rRNA methyltransferase

essential: no

ec: 2.1.1.-

synonyms: yacO, rlmB

List of homologs in different organisms, belongs to COG0566 (Galperin et al., 2021)

Gene

Coordinates: 115,269  116,018

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The protein

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)
• class IV-like SAM-binding methyltransferase superfamily (with [protein|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR] and [protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP], according to UniProt)

[wiki|Cofactors]

• SAM (according to UniProt)

Structure

• [PDB|1GZ0] (from ''E. coli'', 44% identity) [Pubmed|12377117]
• [AF|Q06753]

Paralogous protein(s)

• [protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP]

[wiki|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]

  description: [Pubmed|7510287]

  regulation

  • expression transiently increases in the forespore [Pubmed|22848659]
  • the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • T-box: RNA switch, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|other_regulator:T-box|T-box]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7510287], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B884 (yacO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1883 NBRP B. subtilis, Japan]
• BKE00960 ([gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGCATTTTTCCCTATGA,  downstream forward: _UP4_AACCCTGTGGGAGAATAAAG
• BKK00960 ([gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00960 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTGCATTTTTCCCTATGA,  downstream forward: _UP4_AACCCTGTGGGAGAATAAAG

References

• 36815589,19258532,7510287,12377117,11698387