ureC

168

urease (alpha subunit)

locus: BSU_36640

Molecular weight: 61.01 kDa

pI: 5.09

Protein length: 569 aa

Gene length: 1710 bp

function: utilization of urea as alternative nitrogen source

product: urease (alpha subunit)

essential: no

ec: 3.5.1.5

synonyms: ureC

List of homologs in different organisms, belongs to COG0804 (Galperin et al., 2021)

Gene

Coordinates: 3,766,714 → 3,768,423

Advertisement

The protein

Catalyzed reaction/ biological activity

• 2 H⁺ + H₂O + urea --> CO₂ + 2 NH₄⁺ (according to UniProt)

Protein family

• [wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)

[wiki|Domains]

• Urease domain (aa 132-569) (according to UniProt)

[wiki|Cofactors]

• nickel

Structure

• [PDB|5A6T] (from Sporosarcina pasteurii, 65% identity) [pubmed|26580226]
• [AF|P77837]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|63CF8C8BBCE39BD585DD5F6C8BBD95E12B500AE6|ywzF]-[gene|1D9596609AEF10456041918BD592E49466D93B1F|ywzE]-[gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]

  description: [Pubmed|22383849]

• genes: [gene|57B6D70B2570C308377B301EC2AF6C232233FD46|ureA]-[gene|B1559A1482B1F5932E6F77B32BF87E7474E01C17|ureB]-[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]

  description: [Pubmed|9287005]

  regulation

  • induced by nitrogen limitation ([protein|search|GlnR], [protein|search|TnrA]) [Pubmed|9287005]

  regulatory mechanism

  • [protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, [Pubmed|9287005], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [Pubmed|9287005], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
  • [protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12374841], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|9287005,12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9287005], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|9287005], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]

Biological materials

Mutant

• GP3982 (Δ[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]::aphA3 trpC2), available in [wiki|Jörg Stülke]'s lab
• BKE36640 (Δ[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCATATTCCTCCCGTGACA,  downstream forward: _UP4_TGATAAGACGCGGCCGGAGT
• BKK36640 (Δ[gene|CD3DD65D6EFF11564088CA8D56640F772DEAC2D9|ureC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCATATTCCTCCCGTGACA,  downstream forward: _UP4_TGATAAGACGCGGCCGGAGT

Expression vectors

• pGP3760: expression of Strep-''ureC'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 23539618,19363030

Original Publications

• 12618455,16199586,9287005,9287005,12374841,9287005,25755103,26580226