ung

168

uracil-DNA glycosylase

locus: BSU_37970

Molecular weight: 25.90 kDa

pI: 8.78

Protein length: 225 aa

Gene length: 678 bp

function: DNA repair

product: uracil-DNA glycosylase

essential: no

synonyms: ung, ipa-57d, ywdG

List of homologs in different organisms, belongs to COG0692 (Galperin et al., 2021)

Gene

Coordinates: 3,897,685 → 3,898,362

Phenotypes of a mutant

• increased mutation rates [Pubmed|22056936]

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The protein

Catalyzed reaction/ biological activity

• removes uracil preferentially from single-stranded DNA over double-stranded DNA, exhibiting higher preference for U:G than U:A mismatches [Pubmed|21542855]

Protein family

• uracil-DNA glycosylase (UDG) superfamily (single member, according to UniProt)

Structure

• [PDB|3A7N] (from ''Mycobacterium tuberculosis'', 42% identity) [Pubmed|20693660]
• [AF|P39615]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DC73C11C3C54D35B2D9A0A28666A29E36451E7F8|ung]

  description: [Pubmed|9353933]

  regulation

  • expressed throughout growth and staionary phase [Pubmed|22056936]

• genes: [gene|1DA87B205F2E201DE027209620110D043AF8A6AF|ywdF]-[gene|DC73C11C3C54D35B2D9A0A28666A29E36451E7F8|ung]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE37970 (Δ[gene|DC73C11C3C54D35B2D9A0A28666A29E36451E7F8|ung]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAGCTGTTTCAAGATTCAGG,  downstream forward: _UP4_TAAAAAGCGCAGGTGATCTG
• BKK37970 (Δ[gene|DC73C11C3C54D35B2D9A0A28666A29E36451E7F8|ung]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AAGCTGTTTCAAGATTCAGG,  downstream forward: _UP4_TAAAAAGCGCAGGTGATCTG

References

Reviews

• 22933559,38551349

Original Publications

• 9353933,20693660,22056936,21170359,21542855,25326311,27530626