trmU

168

tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules

locus: BSU_27500

Molecular weight: 42.20 kDa

pI: 4.73

Protein length: 371 aa

Gene length: 1116 bp

function: tRNA modification

product: tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase

essential: yes

ec: 2.8.1.-

synonyms: trmU, yrrA, mnmA

List of homologs in different organisms, belongs to COG0482 (Galperin et al., 2021)

Gene

Coordinates: 2,809,413  2,810,528

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]) [Pubmed|25825430]
• U34 in tRNA + L-cysteine + ATP --> (s²U34 in tRNA) + alanine + AMP + ppi [Pubmed|25825430]

Protein family

• MnmA/TRMU family (single member, according to UniProt)

Structure

• [PDB|2HMA] (from ''Streptococcus pneumoniae'', 66% identity)
• [AF|O35020]

[wiki|Localization]

• cytoplasm(according to UniProt)

Expression and Regulation

Operons

• genes: [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]

  description: [Pubmed|22383849]

• genes: [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]

  description: [Pubmed|16885442]

  regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16885442], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

References

• 16885442,11948165,25825430,35467390