trmD

168

tRNA methyltransferase, methylates G37 residue at the N1 position in a subset of tRNAs

locus: BSU_16030

Molecular weight: 27.56 kDa

pI: 5.31

Protein length: 243 aa

Gene length: 732 bp

function: tRNA modification

product: tRNA methyltransferase

essential: yes

ec: 2.1.1.31

synonyms: trmD

List of homologs in different organisms, belongs to COG0336 (Galperin et al., 2021)

Gene

Coordinates: 1,675,171  1,675,902

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• guanosine³⁷ in tRNA + S-adenosyl-L-methionine --> H⁺ + N¹-methylguanosine³⁷ in tRNA + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• RNA methyltransferase trmD family (single member, according to UniProt)

Structure

• [PDB|3KY7] (from ''Staphylococcus aureus'', 62% identity)
• [AF|O31741]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|8F559BFC80FE56EA0389A2336F4E9E6908562BCB|ylxM]-[gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh]-[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]-[gene|885ECD9FAAC8BC95D32F049825D6B6244C046112|trmD]-[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]

  description: [Pubmed|29794222]

  regulation

  • the mRNA is processed between [gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh] and [gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

  expression

  • expression is decreased upon depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8335643], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|khpA]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]-[gene|885ECD9FAAC8BC95D32F049825D6B6244C046112|trmD]-[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]

  description: [Pubmed|36840557]

References

• 36840557