thiO

168

FAD-dependent glycine oxidase

locus: BSU_11670

Molecular weight: 40.78 kDa

pI: 5.9

Protein length: 369 aa

Gene length: 1110 bp

function: biosynthesis of thiamine

product: glycine oxidase

essential: no

ec: 1.4.3.19

synonyms: thiO, goxB, yjbR

List of homologs in different organisms, belongs to COG0665 (Galperin et al., 2021)

Gene

Coordinates: 1,243,735  1,244,844

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The protein

Catalyzed reaction/ biological activity

• glycine + H₂O + O₂ --> glyoxylate + H₂O₂ + NH₄⁺ (according to UniProt)
• H₂O + N-ethylglycine + O₂ --> ethylamine + glyoxylate + H₂O₂ (according to UniProt)
• H₂O + O₂ + sarcosine --> glyoxylate + H₂O₂ + methylamine (according to UniProt)
• D-alanine + H₂O + O₂ --> H₂O₂ + NH₄⁺ + pyruvate (according to UniProt)
• glyphosate + H₂O + O₂ --> aminomethylphosphonate + glyoxylate + H⁺ + H₂O₂ (according to UniProt)

Protein family

• DAO family (single member, according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|1NG3] (complex with acetyl-glycine),  [PDB|1RYI]
• [AF|O31616]

Modification

• FAD [Pubmed|19751796]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]

  description: [Pubmed|1898926]

  regulation

  • repressed by thiamine ([wiki|Thi-box]) [Pubmed|16356850]
  • the [wiki|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• MGNA-B164 (yjbR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1163 NBRP B. subtilis, Japan]
• BKE11670 ([gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATTCCGCCTCCAATCACCA,  downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT
• BKK11670 ([gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11670 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AATTCCGCCTCCAATCACCA,  downstream forward: _UP4_CGCAAGGAGGCGGTTCAGAT

References

Reviews

• 19348578,10382260

Original Publications

• 19254749,11744710,12654003,9827558,12627963,15105420,19751796,19864430,14567704,20690620,24925096,31562850