thiL

168

thiamine-monophosphate kinase

locus: BSU_05900

Molecular weight: 35.73 kDa

pI: 5.04

Protein length: 325 aa

Gene length: 978 bp

function: biosynthesis of thiamine

product: thiamine-monophosphate kinase

essential: no

ec: 2.7.4.16

synonyms: thiL, ydiA, ydxA

List of homologs in different organisms, belongs to COG0611 (Galperin et al., 2021)

Gene

Coordinates: 640,662 → 641,639

Advertisement

The protein

Catalyzed reaction/ biological activity

• ATP + thiamine phosphate --> ADP + thiamine diphosphate (according to UniProt)

Protein family

• thiamine-monophosphate kinase family (single member, according to UniProt)

Structure

• [PDB|1VQV] (from ''Aquifex aeolicus'', 33% similarity, 51% identity)
• [AF|O05514]

Expression and Regulation

Operons

• genes: [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|rimI]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-C197 (ydiA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2195 NBRP B. subtilis, Japan]
• BKE05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT,  downstream forward: _UP4_TTTAAATAAGACGGGTGACT
• BKK05900 (Δ[gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAACCAAAACCTCCTTT,  downstream forward: _UP4_TTTAAATAAGACGGGTGACT

References

Reviews

• 19348578

Original Publications

• 16291685,22383849