tenA

168

thiaminase II

locus: BSU_11650

Molecular weight: 27.27 kDa

pI: 4.99

Protein length: 236 aa

Gene length: 711 bp

function: thiamine salvage

product: thiaminase II

essential: no

ec: 3.5.99.2

synonyms: tenA

List of homologs in different organisms, belongs to COG0819 (Galperin et al., 2021)

Gene

Coordinates: 1,242,449 1,243,159

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The protein

Catalyzed reaction/ biological activity

• 4-amino-5-aminomethyl-2-methylpyrimidine + H₂O --> 4-amino-5-hydroxymethyl-2-methylpyrimidine + NH₄⁺(according to UniProt)
• H₂O + thiamine --> 4-amino-5-hydroxymethyl-2-methylpyrimidine + 5-(2-hydroxyethyl)-4-methylthiazole + H⁺ (according to UniProt)

Protein family

• TenA family (single member, according to UniProt)

Structure

• [PDB|1YAF], [PDB|2QCX] (complex with formyl aminommethyl pyrimidine)
• [AF|P25052]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• BKE11650 ([gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAATCATTCCCCCTCTG, downstream forward: _UP4_AATGTGGAGCTTCACGCCAT
• BKK11650 ([gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAATCATTCCCCCTCTG, downstream forward: _UP4_AATGTGGAGCTTCACGCCAT

References

Reviews

• 10382260

Original Publications

• 15709744,18054064,17618314,16356850,24311574,15378759,29794222