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  • Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

Gene name ywtF
Essential no
Product phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagT
Interactions involving this protein in SubtInteract: TagT
MW, pI 35 kDa, 9.554
Gene length, protein length 966 bp, 322 aa
Immediate neighbours ywtG, ywtE
Sequences Protein DNA DNA_with_flanks
Genetic context
YwtF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TagT expression.png

Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU35840

Phenotypes of a mutant

  • a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: LytR-Cps2a-Psr family PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed
    • extracellular (signal peptide) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at high salt concentrations (SigM) Pubmed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 105 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 258 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks



Stephanie Brown, John P Santa Maria, Suzanne Walker
Wall teichoic acids of gram-positive bacteria.
Annu Rev Microbiol: 2013, 67;313-36
[PubMed:24024634] [WorldCat.org] [DOI] (I p)

Original publications

Alice Eberhardt, Christopher N Hoyland, Daniela Vollmer, Stephanie Bisle, Robert M Cleverley, Ola Johnsborg, Leiv S Håvarstein, Richard J Lewis, Waldemar Vollmer
Attachment of capsular polysaccharide to the cell wall in Streptococcus pneumoniae.
Microb Drug Resist: 2012, 18(3);240-55
[PubMed:22432711] [WorldCat.org] [DOI] (I p)

Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel, Jeff Errington
A widespread family of bacterial cell wall assembly proteins.
EMBO J: 2011, 30(24);4931-41
[PubMed:21964069] [WorldCat.org] [DOI] (I e)

Judith Hübscher, Lucas Lüthy, Brigitte Berger-Bächi, Patricia Stutzmann Meier
Phylogenetic distribution and membrane topology of the LytR-CpsA-Psr protein family.
BMC Genomics: 2008, 9;617
[PubMed:19099556] [WorldCat.org] [DOI] (I e)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)