ssuB

168

aliphatic sulfonate [wiki|ABC transporter] (binding protein)

locus: BSU_08830

Molecular weight: 30.08 kDa

pI: 8.67

Protein length: 255 aa

Gene length: 768 bp

function: sulfonate uptake

product: aliphatic sulfonate [wiki|ABC transporter] (binding protein)

essential: no

synonyms: ssuB, ygaL

List of homologs in different organisms, belongs to COG1116 (Galperin et al., 2021)

Gene

Coordinates: 961,394  962,161

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 7-231) (according to UniProt)

Structure

• [PDB|1Q1B] (MalK from E. coli, 38% identity) [pubmed|14527411]
• [AF|P97027]

Paralogous protein(s)

• [protein|3DC6A032B0F076A460FE2C3244D747CFC7583FE7|ytlC]

[wiki|Localization]

• associated to the membrane (via [protein|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]-[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]-[gene|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]-[gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]-[gene|93F7A5795A423CD8DD7B070F7F37731A5E8D6321|ygaN]

  description: [Pubmed|11251850]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11251850], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A249 (ygaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/249 NBRP B. subtilis, Japan]
• BKE08830 ([gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC,  downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG
• BKK08830 ([gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC,  downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG

References

• 15937167,11251850,10092453,16513748,9782504,14527411