ssuA

168

aliphatic sulfonate ABC transporter (binding lipoprotein)

locus: BSU_08840

Molecular weight: 36.18 kDa

pI: 4.85

Protein length: 332 aa

Gene length: 999 bp

function: sulfonate uptake

product: aliphatic sulfonate ABC transporter (binding lipoprotein)

essential: no

synonyms: ssuA, ygbA, yzeA

List of homologs in different organisms, belongs to COG0715 (Galperin et al., 2021)

Gene

Coordinates: 962,179 → 963,177

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The protein

Protein family

• bacterial solute-binding protein ssuA/tauA family (with [protein|56E482D9CAC6E350140FD21A1C4AC8820B918CDA|ytlA], according to UniProt)

Structure

• [PDB|2X26] (from E. coli,32% identity ) [pubmed|20383006]
• [AF|P40400]

[wiki|Localization]

• associated to the membrane (via [protein|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]-[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]-[gene|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]-[gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]-[gene|93F7A5795A423CD8DD7B070F7F37731A5E8D6321|ygaN]

  description: [Pubmed|11251850]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11251850], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A252 (ygbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/252 NBRP B. subtilis, Japan]
• 1A800 ( ''ssuA''::''kan''), [Pubmed|9782504], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A800&Search=1A800 BGSC]
• BKE08840 (Δ[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCTTCCGCCCTTT,  downstream forward: _UP4_CAATGATGAAAGCAGAGGCT
• BKK08840 (Δ[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCTTCCGCCCTTT,  downstream forward: _UP4_CAATGATGAAAGCAGAGGCT

References

• 15937167,10092453,16513748,11251850,9782504,20383006