sspD

168

small acid-soluble spore protein (minor alpha/beta-type SASP)

locus: BSU_13470

Molecular weight: 6.67 kDa

pI: 8.94

Protein length: 64 aa

Gene length: 195 bp

function: protection of spore DNA

product: small acid-soluble spore protein (minor alpha/beta-type SASP)

essential: no

synonyms: sspD

List of homologs in different organisms, belongs to COG5852 (Galperin et al., 2021)

Gene

Coordinates: 1,413,800 → 1,413,994

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The protein

Protein family

• [wiki|Alpha/beta-type SASP family] (according to UniProt)

Structure

• [PDB|2Z3X] ([protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|sspC]-DNA complex, 59% identity)
• [AF|P04833]

Paralogous protein(s)

• [protein|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA], [protein|28F47ADECD376494878AE58B395B3A2616B6B5DF|sspB], [protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|sspC]

Expression and Regulation

Operons

• genes: [gene|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD]

  description: [Pubmed|3009398]

  regulation

  • expressed during sporulation in the forespore ([protein|search|SigG], [wiki|SpoVT]) [Pubmed|15699190,8755877]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: activation, [Pubmed|8755877], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

Biological materials

Mutant

• BKE13470 (Δ[gene|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCATCTCCTTTT,  downstream forward: _UP4_GGCACAACTAAATAAATTCA
• BKK13470 (Δ[gene|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTTTTCATCTCCTTTT,  downstream forward: _UP4_GGCACAACTAAATAAATTCA

References

Reviews

• 35638784

Original Publications

• 3009398,15699190,8755877