spoVID

168

developmental checkpoint, co-ordinates spore cortex and coat assembly, spore coat morphogenetic protein, non-competitive hub during spore encasement, promotes encasement of the spore

locus: BSU_28110

Molecular weight: 64.80 kDa

pI: 4

Protein length: 575 aa

Gene length: 1728 bp

function: spore coat assembly

product: spore coat morphogenetic protein

essential: no

synonyms: spoVID

List of homologs in different organisms, belongs to COG5846 (Galperin et al., 2021)

Gene

Coordinates: 2,871,022  2,872,749

Phenotypes of a mutant

• accumulation of [protein|CEBEC9CECCF445C40D793E61A2CD23175631A473|safA] and [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE] and their dependent proteins at the mother cell proximal spore pole [pubmed|30168214]

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The protein

Catalyzed reaction/ biological activity

• measures the successful polymerization of the coat basement layer [pubmed|35065768]
• ensures orchestrated assembly of the spore envelope [pubmed|35065768]

[wiki|Domains]

• contains a N-acetylglucosamine-polymer-binding [wiki|LysM domain] (aa 523-567) [Pubmed|18430080]
• [wiki|LysM domain] (aa 523-567) (according to UniProt)

Structure

• [AF|P37963]

[wiki|Localization]

• spore coat (basement), localization depends on [protein|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA] [Pubmed|22773792,22171814]

Expression and Regulation

Operons

• genes: [gene|6698BB092E03BEF48AFD0CBF565410109CD1ABB5|spoVID]-[gene|D1EAEC9C88EDDD8FB2E7E315DE28A7A54AAAB4D2|ysxE]

  description: [Pubmed|15699190,8449878]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,8449878]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,8449878], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• 1A639 ( ''spoVID''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A639&Search=1A639 BGSC]
• 1S127 ( ''spoVID''::''erm''), [Pubmed|16751597], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S127&Search=1S127 BGSC]
• BKE28110 ([gene|6698BB092E03BEF48AFD0CBF565410109CD1ABB5|spoVID]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTTCATATCCTCCTTTC,  downstream forward: _UP4_TAAACCGTAAGTGATCGGAA
• BKK28110 ([gene|6698BB092E03BEF48AFD0CBF565410109CD1ABB5|spoVID]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTTCATATCCTCCTTTC,  downstream forward: _UP4_TAAACCGTAAGTGATCGGAA

labs

• [wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
• [wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

References

Reviews

• 23202530,18430080,35346552

Original Publications

• 16950916,11325931,12107147,10714986,19775244,15699190,8449878,22773792,22171814,23178679,25259857,19702880,22262582,26187959,30168214,30455281,35065768,37986874