spoIIIE

spoIIIE
168

ATP-dependent dsDNA translocase, resolution of chromosomal dimers after [wiki|DNA replication], coordinates chromosome segregation and peptidoglycan remodeling during sporulation, transports the forespore chromosome across the [wiki|sporulation] septum

locus
BSU_16800
Molecular weight
86.96 kDa
pI
6.76
Protein length
Gene length
function
chromosome partition during [wiki|sporulation]
product
ATP-dependent dsDNA translocase required for chromosome partition
essential
no
synonyms
spoIIIE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1674 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,752,272  1,754,641
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The protein
Catalyzed reaction/ biological activity
transports the forespore chromosome across the [wiki|sporulation ]septum [Pubmed|20516200]
translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes [Pubmed|19818024]
the two DNA translocases [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA] and [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG [Pubmed|18391964]
Protein family
FtsK/SpoIIIE/SftA family (with [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA], according to UniProt)
[wiki|Domains]
N-terminal transmembrane domain (4 helices) [Pubmed|24297254]
134 aa linker [Pubmed|24297254]
512 aa motor domain at the C-terminus [Pubmed|24297254]
[wiki|FtsK domain] (aa 450-646) (according to UniProt)
Structure
[PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]) [Pubmed|16916635]
[AF|P21458]
Paralogous protein(s)
[protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]
[wiki|Localization]
free diffusion in the membrane, no enrichment at septa [pubmed|29439991]
transmembrane domain mediates dynamic localization to active division sites [Pubmed|20516200]
complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [wiki|sporulation] and division septa [Pubmed|23667326]
anchored at the edge of a septal pore, stabilized by newly synthesized peptidoglycan and protein-protein interactions across the septum [pubmed|33383000]
Expression and Regulation
Operons
genes
[gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]-[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2507870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]→[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]

2024-02-15 10:14:40

Jstuelk

55

20931424e7f3957a516f01163ac7a07a7038a992

7D54335218ECF5D963FC72675848DF5C0DA67794

Biological materials
Mutant
BKE16800 ([gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC,  downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
BKK16800 ([gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC,  downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
References
Reviews
22047950,22934648,23400100,25460803,32660383,34728126,35638784
Original Publications
29439991,18160039,18593879,3129532,2507870,18593879,23559069,9150232,2492502,11062134,9155041,14680637,7797072,7567988,2507870,8160014,9515703,19818024,19788545,20298190,20516200,21239579,18391964,23667326,11134515,24297254,24769697,25950186,26452092,16916635,26849443,27886417,27891124,29425492,29504934,29588476,32778559,33383000

125325B485FBC60798EDA4B4BFB24C6FCBC4C513

Page visits: 5153

Time of last update: 2024-03-28 22:39:07

Author of last update: Jstuelk