slrA

168

negative regulator of the [wiki|fla-che operon], anti-repressor, antagonist [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] for the repression of the [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]→[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO] and [gene|8C13E1EF5437DD8F9906643DEDCBB18DABE3B9E3|tapA]-[gene|09B082BF39703F0D9A5980E643175DBD59F5B228|sipW]-[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA] operons

locus: BSU_38229

Molecular weight: 6.00 kDa

pI: 9

Protein length: 52 aa

Gene length: 159 bp

function: control of motility and biofilm formation

product: antagonist of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] and [protein|920F91E748EE079FF864011D9052B073567C41E4|slrR]

essential: no

synonyms: slrA

List of homologs in different organisms

Gene

Coordinates: 3,923,319 → 3,923,477

RNA

additional information

• the mRNA has long 5' and 3' UTRs (170 and 330 nt, respectively) [pubmed|26857544]

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The protein

Catalyzed reaction/ biological activity

• binding of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] resulting in induction of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]-repressed genes and operons [Pubmed|19788541]

[wiki|Domains]

• [wiki|Sin domain] (aa 1-38) (according to UniProt)

Structure

• [AF|P0C8M5]

Effectors of protein activity

• [protein|920F91E748EE079FF864011D9052B073567C41E4|slrR] inhibits activity of [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|slrA] (i. e. binding to [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]) [Pubmed|19788541]

Paralogous protein(s)

• [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|sinI]

Biological materials

Mutant

• BKE38229 (Δ[gene|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|slrA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38229 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTAACCTCCAATTGTA,  downstream forward: _UP4_TAGTCCGAACAGGCGGATCT
• BKK38229 (Δ[gene|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|slrA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38229 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTAACCTCCAATTGTA,  downstream forward: _UP4_TAGTCCGAACAGGCGGATCT

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Stülke] lab

References

• 18647168,19788541,23430750,26857544,22329926,35311531