sipV

168

signal peptidase I

locus: BSU_10490

Molecular weight: 18.81 kDa

pI: 10.12

Protein length: 168 aa

Gene length: 507 bp

function: protein secretion

product: signal peptidase I

essential: no

synonyms: sipV, yhjF

List of homologs in different organisms, belongs to COG0681 (Galperin et al., 2021)

Gene

Coordinates: 1,122,175  1,122,681

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The protein

Catalyzed reaction/ biological activity

• Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins (according to UniProt)

Protein family

• [wiki|peptidase S26 family] (according to Uniprot)

Structure

• [PDB|4NV4] (from B. anthracis, 47% identity)
• [AF|O07560]

Paralogous protein(s)

• [protein|C2EAE417DB9250B88BB0E25D4AE760BDE82EFD86|sipT], [protein|CDC6971F39F3EEAAE912CB1402EE1BD64D5A12A0|sipS], [protein|9824F9CF9C2028AF267AC43FE7FD8590E8231AB8|sipU]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|020DBB58794BF6C450E9A654719E62B2D3B10F04|yhjE]-[gene|ADEEA5E15C100C1DC6E129B9A3E6DECAFCA8C409|sipV]

  description: [Pubmed|22383849]

  regulation

  • induced upon DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

Biological materials

Mutant

• BKE10490 ([gene|ADEEA5E15C100C1DC6E129B9A3E6DECAFCA8C409|sipV]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTACTGTCTCCCTCCA,  downstream forward: _UP4_TAAAAAGACGCTAATTGAAG
• BKK10490 ([gene|ADEEA5E15C100C1DC6E129B9A3E6DECAFCA8C409|sipV]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTACTGTCTCCCTCCA,  downstream forward: _UP4_TAAAAAGACGCTAATTGAAG

labs

• [wiki|Jan Maarten van Dijl], Groningen, Netherlands

References

Reviews

• 22688815,34410368

Original Publications

• 9325333,9694797,16267290,28088521