rpsNB

168

alternative ribosomal protein S14*, can replace [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN] under conditions of zinc limitation

locus: BSU_08880

Molecular weight: 10.20 kDa

pI: 11.19

Protein length: 89 aa

Gene length: 270 bp

function: translation

product: alternative ribosomal protein S14*

essential: no

synonyms: rpsNB, rpsNB, yhzA

List of homologs in different organisms, belongs to COG0199 (Galperin et al., 2021)

Gene

Coordinates: 965,909  966,178

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The protein

Protein family

• universal [wiki|ribosomal protein] uS14 family (with [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN], according to UniProt)

Structure

• [PDB|5AFI] (the E. coli ribosome, 47% identity) [pubmed|25707802]
• [AF|O31587]

Paralogous protein(s)

• [protein|0CC562884650F322298BEA28C889E2ABCB01AFDE|rpsN]

Expression and Regulation

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• BKE08880 ([gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT,  downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC
• BKK08880 ([gene|6212D2DB719ACC477DA835EAC198545318FFEFAC|rpsNB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08880 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTCACATAGCCTCCCTT,  downstream forward: _UP4_TGGTAAGAAAAAACTCCGGC

References

• 17163968,12884008,12904577,20208344,12904577,17163968,19648245,23002217,27561249,25707802