rpsF

168

ribosomal protein bS6

locus: BSU_40910

Molecular weight: 10.99 kDa

pI: 4.99

Protein length: 95 aa

Gene length: 288 bp

function: translation

product: ribosomal protein bS6

essential: no

synonyms: rpsF

List of homologs in different organisms, belongs to COG0360 (Galperin et al., 2021)

Gene

Coordinates: 4,199,445 4,199,732

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bS6 family (single member, according to UniProt)

Structure

• [PDB|3R3T] (from ''Bacillus anthracis'', 71% identity, 97% similarity)
• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P21468]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]
• this ribosomal protein is lacking in some organisms with very small genomes [pubmed|33753464]

Expression and Regulation

Operons

• genes: [gene|BD162A17E5F3F9AB49E478CE72D0FE92D82E6673|yyzM]-[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]-[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]

  description: [Pubmed|22383849]

• genes: [gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]

  description: [Pubmed|14762004]

  regulation

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24310371], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]

  description: [Pubmed|24310371,22383849]

  regulation

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]

Biological materials

Mutant

• BKE40910 ([gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTGCACCTCCTTT, downstream forward: _UP4_TAAGCAATTTTGAAATATAT
• BKK40910 ([gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTTGCACCTCCTTT, downstream forward: _UP4_TAAGCAATTTTGAAATATAT

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

References

• 36815589,19653700,14762004,11948165,11948146,23002217,22383849,24310371,15378759,25903689,33753464