rpmB

168

ribosomal protein bL28

locus: BSU_15820

Molecular weight: 6.67 kDa

pI: 12.24

Protein length: 62 aa

Gene length: 189 bp

function: translation

product: ribosomal protein L28 (bL28)

essential: no

synonyms: rpmB, yloT

List of homologs in different organisms, belongs to COG0227 (Galperin et al., 2021)

Gene

Coordinates: 1,655,599  1,655,787

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bL28 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P37807]

Additional information

• the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA] [Pubmed|24335279,23700310]

Expression and Regulation

Operons

• genes: [gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|rpmB]

  description: [pubmed|22383849]

  regulation

  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

  regulatory mechanism

  • stringent response: negative regulation, [Pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• BKE15820 ([gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|rpmB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTCCCTCCTCACT,  downstream forward: _UP4_TAACAAAATGAACGCCTGCC
• BKK15820 ([gene|A58851E0B5C4DE4D6F3C16FF8C009001E393C3B8|rpmB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGTTTCCCTCCTCACT,  downstream forward: _UP4_TAACAAAATGAACGCCTGCC

References

• 19653700,23002217,23033921,23700310,24335279,25903689,33753464,33782055