xerD

168

site-specific integrase/recombinase, partitioning of the terminus region after replication, terminus-specific unloader of translocating SMC complexes

locus: BSU_23510

Molecular weight: 33.84 kDa

pI: 10

Protein length: 296 aa

Gene length: 891 bp

function: chromosome partitioning, unloading of SMC complexes

product: site-specific integrase/recombinase

essential: no

synonyms: ripX, ykqM

List of homologs in different organisms, belongs to COG4974 (Galperin et al., 2021)

Gene

Coordinates: 2,448,591  2,449,481

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The protein

Catalyzed reaction/ biological activity

• resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)
• functions as a site-specific unloader of translocating [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complexes at the replication terminus [pubmed|33472056]

Protein family

• [wiki|phage integrase family] (according to UniProt)

Structure

• [PDB|1A0P] (from ''E. coli'', 44% identity, complex with [protein|89746E312F20C81BE3CFDFCE7D99B01C375C198B|codV]) [Pubmed|9311978]
• [AF|P46352]

Paralogous protein(s)

• [protein|89746E312F20C81BE3CFDFCE7D99B01C375C198B|codV]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)
• assembles on the chromosome at the dif site throughout the cell cycle [Pubmed|21239579]

Biological materials

Mutant

• MGNA-C414 ([gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|xerD]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2412 NBRP B. subtilis, Japan]
• 1A793 ( [gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|xerD]::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A793&Search=1A793 BGSC]
• 1A796 ([gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|xerD]::''spec''), [Pubmed|11208805], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A796&Search=1A796 BGSC]
• BKE23510 ([gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|xerD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTATTTGATCTTTCACTC,  downstream forward: _UP4_TAGCCGCAGGTGGCTGCGGC
• BKK23510 ([gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|xerD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTATTTGATCTTTCACTC,  downstream forward: _UP4_TAGCCGCAGGTGGCTGCGGC

References

Reviews

• 23400100

Original Publications

• 10498718,16597952,11208805,21239579,11134515,9311978,33472056