rhaB

168

rhamnulokinase

locus: BSU_31200

Molecular weight: 54.57 kDa

pI: 5.42

Protein length: 485 aa

Gene length: 1458 bp

function: utilization of rhamnose

product: rhamnulokinase

essential: no

ec: 2.7.1.5

synonyms: rhaB, yulC

List of homologs in different organisms, belongs to COG1070 (Galperin et al., 2021)

Gene

Coordinates: 3,199,565  3,201,022

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The protein

Catalyzed reaction/ biological activity

• L-rhamnulose + ATP --> L-rhamnulose-1-phosphate + ADP [Pubmed|26712933]

Protein family

• rhamnulokinase family (single member, according to UniProt)

Structure

• [PDB|2UYT] (the enzyme of ''E. coli'')
• [AF|O05262]

Expression and Regulation

Operons

• genes: [gene|C32AE30BF7931D6171E9DE75C67504F81DD0F227|rhaEW]-[gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]

  description: [Pubmed|26712933,22383849]

  regulation

  • expression during spore [wiki|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

  regulatory mechanism

  • [protein|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]: repression, [Pubmed|26712933], in [regulon|protein:F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|26712933], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|26712933], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|F574142C1E61788FF8F4B61280AD4F87292D08DF|rhaR]-[gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]-[gene|294C0CC675088B2A2EEFC26FAF1A628002B82C59|rhaM]-[gene|FDDC16D57752D81299CDD426257C1CD49890C89C|rhaA]

  description: [Pubmed|22383849]

  regulation

  • expression during spore [wiki|germination] is increased under conditions of osmotic stress [Pubmed|27766092]

Biological materials

Mutant

• MGNA-B545 (yulC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1544 NBRP B. subtilis, Japan]
• BKE31200 ([gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCAATGGCAGTATAAATCA,  downstream forward: _UP4_AAATGATGAAGAGGTGAAAA
• BKK31200 ([gene|693040A5BA7A25AD485B7B28DBD6E8AAC77450F7|rhaB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31200 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATCAATGGCAGTATAAATCA,  downstream forward: _UP4_AAATGATGAAGAGGTGAAAA

References

• 26712933,16674975,24391637,22383849