recN

168

DNA repair and genetic recombination

locus: BSU_24240

Molecular weight: 64.31 kDa

pI: 4.8

Protein length: 576 aa

Gene length: 1731 bp

function: DNA repair and genetic recombination

product: DNA integrity scanning protein

essential: no

synonyms: recN

List of homologs in different organisms, belongs to COG0497 (Galperin et al., 2021)

Gene

Coordinates: 2,520,557  2,522,287

Phenotypes of a mutant

• reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]

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The protein

Catalyzed reaction/ biological activity

• RecN stimulates polymerizing activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|pnpA] [Pubmed|21859751]

Protein family

• recN family (single member, according to UniProt)

Structure

• [PDB|4AD8] (from Deinococcus radiodurans, 30% identity) [pubmed|23085075]
• [AF|P17894]

[wiki|Localization]

• nucleoid (multiple) [Pubmed|16479537]
• evenly distributed in growing cells [Pubmed|21859751]
• upon DNA damage, [protein|5680A06A74D3EC6310EF8677795511C75DB3059E|recN] forms a single focus per nucleoid [Pubmed|21859751], recruitment is affected by the presence of [protein|A3A2EF3C95B833A11843553107D781EDEFF0BC41|fumarase] and depends on [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] and [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-mediated end resection [pubmed|34339298,29140245]

Expression and Regulation

Operons

• genes: [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]

  description: [Pubmed|2106508]

  regulation

  • by [protein|search|sRNA] [protein|search|sr1]

  additional information

  • expression is fourfold increased upon depletion of ''[wiki|nusA]'' [http://www.nature.com/articles/nmicrobiol20157 Reference]

• genes: [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]

  description: [pubmed|22383849]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

  regulatory mechanism

  • stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• 1A891 ( ''recN''::''cat''), [Pubmed|8510642], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A891&Search=1A891 BGSC]
• BKE24240 ([gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACAGCATTACACCTCTT,  downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA
• BKK24240 ([gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24240 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAAACAGCATTACACCTCTT,  downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA

labs

• [wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

References

Reviews

• 19308706,21517913,22933559,23380520,32286623

Original Publications

• 15849320,19060143,16385024,17999999,2106508,19730681,16479537,21859751,24373815,15186413,8510642,29140245,23085075,30192981,30401797,34339298,36574412