PurR

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  • Description: transcription repressor of the pur operon

Gene name purR
Synonyms yabI
Essential no
Product transcription repressor of the pur operon
Function regulation of purine biosynthesis
Gene expression levels in SubtiExpress: purR
Interactions involving this protein in SubtInteract: PurR
Metabolic function and regulation of this protein in SubtiPathways:
purR
MW, pI 31 kDa, 5.771
Gene length, protein length 855 bp, 285 aa
Immediate neighbours ispE, yabJ
Sequences Protein DNA DNA_with_flanks
Genetic context
PurR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurR expression.png

Contents




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PurR regulon

The PurR regulon

The gene

Basic information

  • Locus tag: BSU00470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Biological activity: transcriptional repressor of purine biosynthetic operons
  • Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

  • Sigma factor:
  • Regulation: negative autoregulation, induction in the presence of PRPP PubMed
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Regulatory mechanism: repression by PurR PubMed
    • PurR: transcription repression PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 301 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 250 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1084 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 845 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1229 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shuobo Shi, Tao Chen, Zhigang Zhang, Xun Chen, Xueming Zhao
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metab. Eng.: 2008, 11(4-5);243-52
[PubMed:19446032] [WorldCat.org] [DOI] (I p)

Per Nygaard, Hans H Saxild
The purine efflux pump PbuE in Bacillus subtilis modulates expression of the PurR and G-box (XptR) regulons by adjusting the purine base pool size.
J. Bacteriol.: 2005, 187(2);791-4
[PubMed:15629952] [WorldCat.org] [DOI] (P p)

Pekka Rappu, Terhi Pullinen, Pekka Mäntsälä
In vivo effect of mutations at the PRPP binding site of the Bacillus subtilis purine repressor.
J. Bacteriol.: 2003, 185(22);6728-31
[PubMed:14594850] [WorldCat.org] (P p)

Aloke Kumar Bera, Jianghai Zhu, Howard Zalkin, Janet L Smith
Functional dissection of the Bacillus subtilis pur operator site.
J. Bacteriol.: 2003, 185(14);4099-109
[PubMed:12837784] [WorldCat.org] (P p)

Sangita C Sinha, Joseph Krahn, Byung Sik Shin, Diana R Tomchick, Howard Zalkin, Janet L Smith
The purine repressor of Bacillus subtilis: a novel combination of domains adapted for transcription regulation.
J. Bacteriol.: 2003, 185(14);4087-98
[PubMed:12837783] [WorldCat.org] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J. Bacteriol.: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

M Weng, H Zalkin
Mutations in the Bacillus subtilis purine repressor that perturb PRPP effector function in vitro and in vivo.
Curr. Microbiol.: 2000, 41(1);56-9
[PubMed:10919400] [WorldCat.org] (P p)

P Rappu, B S Shin, H Zalkin, P Mäntsälä
A role for a highly conserved protein of unknown function in regulation of Bacillus subtilis purA by the purine repressor.
J. Bacteriol.: 1999, 181(12);3810-5
[PubMed:10368157] [WorldCat.org] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc. Natl. Acad. Sci. U.S.A.: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] (P p)

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