purN

168

phosphoribosylglycinamide formyltransferase, irreversible

locus: BSU_06510

Molecular weight: 21.63 kDa

pI: 6.18

Protein length: 195 aa

Gene length: 588 bp

function: purine biosynthesis

product: phosphoribosylglycinamide formyltransferase

essential: no

ec: 2.1.2.2

synonyms: purN

List of homologs in different organisms, belongs to COG0299 (Galperin et al., 2021)

Gene

Coordinates: 708,010  708,597

Advertisement

The protein

Catalyzed reaction/ biological activity

• (6S)-10-formyltetrahydrofolate + N¹-(5-phospho-D-ribosyl)glycinamide --> (6S)-5,6,7,8-tetrahydrofolate + H⁺ + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide (according to UniProt)

Protein family

• GART family (single member, according to UniProt)

Structure

• [PDB|3P9X] (from ''B. halodurans'', 51% identity, 70% similarity)
• [AF|P12040]

Paralogous protein(s)

• [protein|9A399CAA672C3DF90531A7A7168916DE86EFA365|ykkE]

Biological materials

Mutant

• BKE06510 ([gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGATGCAAATACCGCAAACT,  downstream forward: _UP4_TTAAATAACAGAGGTGAAAA
• BKK06510 ([gene|7B30F27D6C459437967A64467E52961F4A9E0F2C|purN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGATGCAAATACCGCAAACT,  downstream forward: _UP4_TTAAATAACAGAGGTGAAAA

References

• 8121396,3036807,12923093,19574646,7638212