pucJ

168

uric acid permease

locus: BSU_32430

Molecular weight: 46.94 kDa

pI: 9.64

Protein length: 449 aa

Gene length: 1350 bp

function: purine utilization

product: uric acid permease

essential: no

synonyms: pucJ, yunJ

List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)

Gene

Coordinates: 3,330,502  3,331,851

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The protein

Protein family

• [wiki|xanthine/uracil permease family] (according to UniProt)
• [wiki|Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily] (according to UniProt)

Structure

• [PDB|3QE7] (E. coli uracil transporter, 31% identity) [pubmed|21423164]
• [AF|O32139]

Paralogous protein(s)

• [protein|7D56F85FBD3AAC66607DD7465592EEAE09D61C98|pbuX], [protein|3162EF36F4441A1E4EBBFDAD19F6768D8EF21B29|pucK]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A935 (yunJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/935 NBRP B. subtilis, Japan]
• BKE32430 ([gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA,  downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
• BKK32430 ([gene|53950BFDC128DE46F3F43BDB9D0FDB3D645152DF|pucJ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTATAGTCCCTCCCTGTA,  downstream forward: _UP4_TTAGAAAAAGAGGTGTAAAA
• BP1257([gene|search|pucJK]::cat trp+) available in [wiki|Jörg Stülke]'s & [wiki|Fabian Commichau]'s lab

References

• 11344136,12823818,12029039,25755103,21423164